Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.This book covers recent advances in hyphenated and multidimensional chromatographic techniques for elucidating the structure, composition, molecular weight, and branching distributions in complex polymers.It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic.This book shows how these techniques provide useful molar mass, architectural, compositional, and thermodynamic information whether SEC is used as the sole separation method or as part of a two-dimensional chromatographic set-up.In the latter case, the ability to separate analytes based on size, provided by SEC, is augmented by separation based on chemical composition, block length, etc., provided by high performance liquid chromatography, liquid chromatography at the critical condition, phase fluctuation chromatography, or high osmotic pressure chromatography.the column calibration, significantly improves reproducibility of SEC measurements and provides much additional information.
These methods are important primarily in polymer science, but also in advanced materials science and emerging technologies.
This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance.
This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.
Addresses special topics in size exclusion chromatography, including characterization of copolymers, inverse size exclusion chromatography, aggregating polymers, polysaccharides, and proteins.
Covers current developments in sedimentation and thermal field flow fractionation for particle size analysis, and characterization of polymer molecular-weight distribution.